Using of Coffee and Cardamom Mixture to Ameliorate Oxidative Stress Induced in ?-irradiated Rats

Human exposure to ionizing radiation induced overproduction of free radicals leading to oxidative stress. This study aimed to evaluate the possibility of using of coffee and cardamom mixture; as natural antioxidant compounds ; to ameliorate oxidative stress in rats induced by exposure to ionizing radiation. Phenolic contents in coffee and essential oils in cardamom were identified by using HPLC chromatography and GC/MS analysis. Four groups of adult male rats were used; the control group (A), the second group (B) received orally the mixture extract of coffee and cardamom (60 mg/100g body weight) for 8weeks, the third group (C) ?irradiated (6 Gy) and the fourth group (D) received orally the mixture extract for 8 weeks and exposed to ?-radiation at the 4 week. The results revealed that the administration of mixture extract of coffee and cardamom to rats significantly reduced the damage effect induced by ?-irradiation via the adjustment of the antioxidant status, decreasing of malondialdehyde content and the subsequent amending of different biochemical parameters as well as some hormones. Accordingly, it is possible to indicate that coffee-cardamom reduced the radiation exposure induced oxidative stress.